SP1 抗体 (Ser101)
Quick Overview for SP1 抗体 (Ser101) (ABIN6972783)
抗原
See all SP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser101
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产品特性
- Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The Sp1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair. Sp1 non-phospho antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.
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纯化方法
- Affinity Purified
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免疫原
- This Sp1 antibody was raised against a peptide containing serine 101 of human Sp1.
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亚型
- IgG
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anti-Sp1 Transcription Factor (SP1) (pThr453) antibody
SP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (C-Term) antibodySP1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 89-198) antibodySP1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4H6 unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 522-618) antibodySP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B11 unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 89-198) antibodySP1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A5 unconjugated
anti-Sp1 Transcription Factor (SP1) antibodySP1 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 89-199) antibodySP1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4H6 unconjugated
anti-Sp1 Transcription Factor (SP1) (pThr739) antibodySP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 420-620) antibodySP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 677-707), (C-Term) antibodySP1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB17350 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- SP1 (Sp1 Transcription Factor (SP1))
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别名
- Sp1
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分子量
- 96 kDa
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NCBI登录号
- NP_612482
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途径
- Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction
抗原
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