Rpb1 CTD 抗体 (pSer5)
Quick Overview for Rpb1 CTD 抗体 (pSer5) (ABIN6972642)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer5
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产品特性
- RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD phospho Ser5 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
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纯化方法
- Protein A Chromatography
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免疫原
- This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.
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亚型
- IgG
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anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pThr4) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Hamster, Schizosaccharomyces pombe WB, IF, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-RNA Polymerase II CTD (pSer7) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated
anti-RNA Polymerase II CTD (pTyr1) antibody适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
anti-RNA Polymerase II CTD (pSer2), (Ser2) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody适用: 人 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E8 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Rpb1 CTD (RNA Polymerase II CTD)
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别名
- RNA pol II CTD
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分子量
- 210 kDa
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NCBI登录号
- NP_000928
抗原
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