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- 抗原 See all Rpb1 CTD products
- Rpb1 CTD (RNA Polymerase II CTD)
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抗原表位
- pSer2
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适用
- 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Rpb1 CTD antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 产品特性
- RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation. RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.
- 纯化方法
- Protein G Chromatography
- 免疫原
- This antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.
- 克隆位点
- 3E7C7
- 亚型
- IgG2a
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anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pTyr1) antibody适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
anti-RNA Polymerase II CTD (pSer2), (Ser2) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer2), (Ser2) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E10 unconjugated
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody适用: 人 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E8 unconjugated
anti-RNA Polymerase II CTD (C-Term) antibody适用: 人, 小鼠, 犬, 大鼠, 斑马鱼, 猴 WB, IF, IHC, IC 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (C-Term), (pSer5) antibody适用: 人, 小鼠, 犬, 大鼠, 斑马鱼 WB, IF, IHC, IC 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (Ser7) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated
anti-RNA Polymerase II CTD (Ser5) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated
anti-RNA Polymerase II CTD (Ser2) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- 抗原
- Rpb1 CTD (RNA Polymerase II CTD)
- 别名
- RNA pol II CTD (Rpb1 CTD 产品)
- 分子量
- 240 kDa
- NCBI登录号
- NP_000928
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