Histone H2A 抗体 (pThr120)
Quick Overview for Histone H2A 抗体 (pThr120) (ABIN6971791)
抗原
See all Histone H2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr120
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产品特性
- Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Phosphorylation of histones occurs at multiple sites during mitosis. H2A Thr120 phosphorylation is observed on chromatin during both mitosis and meiosis. Thr120 phosphorylation is inversely correlated with ubiquitylation of H2A Lys119 in meiotic mouse spermatocytes. In Drosophila, loss of H2A Thr120 phosphorylation is associated with a failure to disassemble the synaptonemal complex, impaired loading of condensin and female infertility. It is possible that H2A Thr120 phosphorylation is involved in the regulation of chromatin structure. Histone H2AT120ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
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纯化方法
- Protein A Chromatography
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免疫原
- This Histone H2A phospho Thr120 antibody was raised against a peptide containing phospho Thr120 of human histone H2A.
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亚型
- IgG
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anti-Histone H2A (pSer139) antibody
适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Histone H2A (acLys5) antibody适用: 人, 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (acLys9) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (pSer139) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (pSer129) antibody适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (meGln104) antibody适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (ubLys119) antibody适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A (pThr121) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H2A antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Histone H2A (pSer129) antibody适用: Saccharomyces cerevisiae WB, DB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Histone H2A
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分子量
- 14 kDa
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NCBI登录号
- NP_003508
抗原
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