Histone H1 抗体 (AA 109-256)
Quick Overview for Histone H1 抗体 (AA 109-256) (ABIN6971739)
抗原
See all Histone H1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 109-256
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产品特性
- The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression. Histone H1 antibody (mAb) (Clone 1G6-E3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Drosophila samples.
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This region of the protein is not conserved in mammals, the antibody is not likely to cross-react to human, mouse or rat Histone H1.
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亚型
- IgG1
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anti-Histone H1 antibody
适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal 1415-1 unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM, CUT&RUN 宿主: 小鼠 Monoclonal AE-4 unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal r1415-1 unconjugated Recombinant Antibody
anti-Histone H1 (AA 1-105) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Monoclonal HH1-1784R unconjugated Recombinant Antibody
anti-Histone H1 (acLys52) antibody适用: 人, 小鼠, 大鼠 FACS, IHC (p), ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 150-207) antibody适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (Lys1535) antibody适用: 人, 小鼠 IF, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 1-194) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Histone H1
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分子量
- 35 kDa
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NCBI登录号
- NP_001027286
抗原
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