BUB1B 抗体 (AA 1-350)
Quick Overview for BUB1B 抗体 (AA 1-350) (ABIN6971476)
抗原
See all BUB1B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-350
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产品特性
- BubR1 (also known as BUB1B, MAD3L and SSK1) is an essential component of the mitotic spindle integrity checkpoint and is required for the normal progression of mitosis, and thus can act as a tumor suppressor. It encodes a serine / threonine kinase localized to the kinetochore and is involved in spindle checkpoint function. BubR1 plays a role in the inhibition of the anaphase-promoting complex (APC), delaying the onset of anaphase and ensuring proper chromosome segregation. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle and the chromosomes are aligned on the metaphase plate. Another function involves the monitoring of kinetochore activities that depend on the kinetochore motor CENP-E. It is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. BubR1 is autophosphorylated, and BubR1 kinase activity is stimulated by association with the centromere protein CENP-E. Phosphorylation at Ser670 and Ser1043 occurs at kinetochores upon mitotic entry with dephosphorylation occurring at the onset of anaphase. Impaired spindle checkpoint function has been found in many forms of cancer. Defects in BubR1 are involved in mosaic variegated aneuploidy syndrome (MVA) and premature chromatid separation trait (PCS). BubR1 interacts with CENP-E, CENP-F, mitosin, PLK1, CASC5, BUB3 and the anaphase-promoting complex (APC). It is part of a complex containing BUB3 and CDC2. BubR1 antibody (mAb) (Clone 8G1) was raised in a Mouse host. It has been validated for use in Immunocytochemistry and Immunofluorescence, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
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纯化方法
- Protein G Chromatography
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免疫原
- This BubR1 antibody was raised against recombinant protein corresponding to amino acids 1-350 of human BubR1.
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亚型
- IgG
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anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibody
BUB1B 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 大鼠, 猴, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 11G2 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 230-394) antibodyBUB1B 适用: 人 IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 274-525) antibodyBUB1B 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, IHC, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (C-Term) antibodyBUB1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 小鼠 WB, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8G1 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 751-850) antibodyBUB1B 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
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别名
- BubR1
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分子量
- 130 kDa
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NCBI登录号
- NP_001202
抗原
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