IL-21 抗体
(2 references)Quick Overview for IL-21 抗体 (ABIN6964031)
抗原
See all IL-21 (IL21) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native and recombinant IL-21
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Recombinant human IL-21
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亚型
- IgG1
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anti-Interleukin 21 (IL21) (AA 25-162) antibody
IL21 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) (AA 1-162) antibodyIL21 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) (AA 65-155) antibodyIL21 适用: 人, 小鼠 WB, ELISA, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) (AA 25-146) antibodyIL21 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) (AA 25-162) antibodyIL21 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) (AA 23-155) antibodyIL21 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) antibodyIL21 适用: 人, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) (AA 1-146) antibodyIL21 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 21 (IL21) (AA 63-162) antibodyIL21 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Neutralization of human IL-21 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: The neutralizing capacity and ND50 was determined using ELISA and ELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/mL) with or without serial dilutions of mAbs MT216G/21.3m. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/mL) with or without serial dilutions of mAbs MT216G/21.3m. After 4 days the IgG-secreting B cells were quantified by ELISpot. Neutralization dose 50 (ND50): MT216G/21.3m neutralized the effect of IL-21 at a ND50value of approximately 0.05 μg/mL when HEK cells were activated with 10 ng/mL of IL-21 (A). The corresponding ND50value for human PBMC activated with 10 ng/mL IL-21 was approximately 0.2 μg/mL. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- supplied at 1 mg/mL in PBS free of preservatives
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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: "Th9 cells promote antitumor immunity via IL-9 and IL-21 and demonstrate atypical cytokine expression in breast cancer." in: International immunopharmacology, Vol. 52, pp. 163-167, (2018) (PubMed).
: "ELISpot and ELISA analyses of human IL-21-secreting cells: Impact of blocking IL-21 interaction with cellular receptors." in: Journal of immunological methods, Vol. 417, pp. 60-66, (2015) (PubMed).
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: "Th9 cells promote antitumor immunity via IL-9 and IL-21 and demonstrate atypical cytokine expression in breast cancer." in: International immunopharmacology, Vol. 52, pp. 163-167, (2018) (PubMed).
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- IL-21 (IL21) (Interleukin 21 (IL21))
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别名
- IL21
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基因ID
- 59067
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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