Interleukin 17a 抗体 (FITC)
Quick Overview for Interleukin 17a 抗体 (FITC) (ABIN6963909)
抗原
See all Interleukin 17a (IL17A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Native and recombinant IL-17A.
-
交叉反应 (详细)
- The antibody also cross-reacts with IL-17A from cow, sheep, horse, pig and dog.
-
纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
-
免疫原
- Recombinant human IL-17A
-
亚型
- IgG1
-
-
-
-
应用备注
- Detection of IL-17A producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-17A by flow cytometry in human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-human IL-17A mAb MT504-FITC and anti-human IFN-γ-PE (right panel). The left panel depicts stimulated cells stained with matched isotype control antibodies. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a BD FACSVerse system.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
-
有效期
- 18 months
-
-
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
-
别名
- IL17A
-
基因ID
- 3605, 282863, 100034142
抗原
-