Perforin 1 抗体 (FITC)
Quick Overview for Perforin 1 抗体 (FITC) (ABIN6963875)
抗原
See all Perforin 1 (PRF1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native Perforin.
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交叉反应 (详细)
- The antibody also cross-reacts with Perforin from pig.
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Native human Perforin
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亚型
- IgG1
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应用备注
- Detection of Perforin producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of Perforin by flow cytometry in viable human peripheral blood mononuclear cells (PBMC). Human PBMC were fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with Pf-344-FITC (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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- Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
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别名
- Perforin
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基因ID
- 5551, 396595
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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