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IL-4 抗体 (PromoFluor 647 Premium)

IL4 适用: 人, Non-Human Primate FACS 宿主: 大鼠 Monoclonal IL4-3 PromoFluor 647 Premium
产品编号 ABIN6963828
发货至: 中国
  • 抗原 See all IL-4 (IL4) 抗体
    IL-4 (IL4) (Interleukin 4 (IL4))
    适用
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    人, Non-Human Primate
    宿主
    • 109
    • 59
    • 55
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    大鼠
    克隆类型
    • 125
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    单克隆
    标记
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    This IL-4 antibody is conjugated to PromoFluor 647 Premium
    应用范围
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    Flow Cytometry (FACS)
    特异性
    Native IL-4
    交叉反应 (详细)
    The monoclonal antibody cross-reacts with IL-4 from non-human primates.
    纯化方法
    Purified from in vitro cultures by protein G affinity chromatography.
    免疫原
    Recombinant human IL-4
    克隆位点
    IL4-3
    亚型
    IgG1
    Top Product
    Discover our top product IL4 Primary Antibody
  • 应用备注
    Detection of IL-4 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-4 by flow cytometry in viable human PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with anti-CD3-BV421, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with PF647P-conjugated mAb IL4-3 (right panel). Non-stimulated cells shown as reference (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    in PBS with 0.2 % BSA and 0.09 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
    有效期
    18 months
  • 抗原
    IL-4 (IL4) (Interleukin 4 (IL4))
    别名
    IL4 (IL4 产品)
    别名
    IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
    基因ID
    3565
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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