Interferon gamma 抗体 (PromoFluor 647 Premium)
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北京 101111
Quick Overview for Interferon gamma 抗体 (PromoFluor 647 Premium) (ABIN6963791)
抗原
See all Interferon gamma (IFNG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native and recombinant porcine IFN-γ
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Recombinant porcine IFN-gamma
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亚型
- IgG2a
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anti-Interferon gamma (IFNG) (AA 75-155) antibody
IFNG 适用: 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 81-166) antibodyIFNG 适用: 人 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 23-156) antibodyIFNG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) antibody (Biotin)IFNG 适用: 人, Non-Human Primate WB, ELISA, IP, FACS, ICC, IHC (p) 宿主: 小鼠 Monoclonal 4S-B3 Biotin
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IFNG-466 unconjugated
anti-Interferon gamma (IFNG) antibodyMicroarray verified IFNG 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB393378 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人, Non-Human Primate WB, ELISA, IP, FACS, ICC, IHC (p), RIA 宿主: 小鼠 Monoclonal 4S-B3 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal F2E4 unconjugated
anti-Interferon gamma (IFNG) (AA 24-166) antibodyIFNG 适用: 豚鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Interferon gamma (IFNG) (AA 23-156) antibodyIFNG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal B7 unconjugated
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应用备注
- Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable porcine PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with CD3-PE and P2C11-PF647P (right panel). The left panel depicts cells stained with a matched isotype control antibody. The dot plots derive from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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- Interferon gamma (IFNG)
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别名
- IFNG
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基因ID
- 396991
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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