Interferon gamma 抗体 (PromoFluor 647 Premium)
(1 reference)Quick Overview for Interferon gamma 抗体 (PromoFluor 647 Premium) (ABIN6963777)
抗原
See all Interferon gamma (IFNG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native and recombinant IFN-γ.
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交叉反应 （详细）
- The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Recombinant bovine IFN-gamma
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亚型
- IgG2a
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anti-Interferon gamma (IFNG) (AA 75-155) antibody
IFNG 适用: 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 81-166) antibodyIFNG 适用: 人 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 23-156) antibodyIFNG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) antibody (Biotin)IFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal 4S-B3 Biotin
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IFNG-466 unconjugated
anti-Interferon gamma (IFNG) antibodyMicroarray verified IFNG 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB393378 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p), RIA 宿主: 小鼠 Monoclonal 4S-B3 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal F2E4 unconjugated
anti-Interferon gamma (IFNG) antibody (APC)IFNG 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal XMG1-2 APC
anti-Interferon gamma (IFNG) (AA 24-166) antibodyIFNG 适用: 豚鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated
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应用备注
- Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable bovine PBMC. Cells were stimulated for 16 hours in the presence of PHA and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT307-PF647P (left panel). The specificity of MT307-PF647P was demonstrated by pre-incubating the antibody with recombinant bovine IFN-γ prior to staining of PBMC (middle panel). Matched isotype control antibody (right panel). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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: "Production of cattle lacking prion protein." in: Nature biotechnology, Vol. 25, Issue 1, pp. 132-8, (2007) (PubMed).
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: "Production of cattle lacking prion protein." in: Nature biotechnology, Vol. 25, Issue 1, pp. 132-8, (2007) (PubMed).
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- Interferon gamma (IFNG)
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别名
- IFNG
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基因ID
- 281237, 443396
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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