Interferon gamma 抗体 (FITC)
Quick Overview for Interferon gamma 抗体 (FITC) (ABIN6963776)
抗原
See all Interferon gamma (IFNG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Native and recombinant IFN-γ.
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交叉反应 (详细)
- The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.
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纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
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免疫原
- Recombinant bovine IFN-gamma
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亚型
- IgG2a
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应用备注
- Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in bovine peripheral blood mononuclear cells (PBMC). Bovine PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-bovine IFN-γ mAb MT307-FITC (right panel). The left panel depicts stimulated cells stained with a matched isotype control antibody. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a Guava EasyCyte Mini.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
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有效期
- 18 months
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- Interferon gamma (IFNG)
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别名
- IFNG
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基因ID
- 281237, 443396
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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