Recombinant mNeonGreen 抗体 (Alexa Fluor 647)
This anti- antibody is a Alpaca 单克隆 antibody detecting in ICC, IF 和 FACS. Suitable for Branchiostoma lanceolatum.
Quick Overview for Recombinant mNeonGreen 抗体 (Alexa Fluor 647) (ABIN6953270)
抗原
mNeonGreen
抗体类型
Recombinant Antibody
片段
single-domain Antibody (sdAb)
适用
Branchiostoma lanceolatum
宿主
Alpaca
克隆类型
单克隆
标记
Alexa Fluor 647
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
克隆位点
1E2
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Expression System
- E.coli
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原理
- Camelid sdAb anti-mNeonGreen conjugated with Alexa647, Clone 1E2
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特异性
- Recognizes mNeonGreen
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产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
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纯化方法
- Produced in: E.coli
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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应用备注
- Recommended dilution 1:500
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说明
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.
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缓冲液
- lyophilized from PBS pH7.4 with 2% BSA (US-Origin)
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注意事项
- Protect from light!
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储存条件
- -20 °C,-80 °C
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储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
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有效期
- 12 months
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- mNeonGreen
抗原
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