Recombinant GFP 抗体 (Alexa Fluor 647)
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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
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适用
- Aequorea victoria
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宿主
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Alpaca
- Expression System
- E.coli
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克隆类型
- 单克隆
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标记
- This GFP antibody is conjugated to Alexa Fluor 647
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应用范围
- Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 原理
- Camelid sdAb anti-GFP conjugated with Alexa647, Clones 1H1/1B2
- 特异性
- Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.
- 交叉反应 （详细）
- Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
- 产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- 纯化方法
- Produced in: E.coli
- 克隆位点
- 1H1-1B2
- Labeling Ratio
- A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.
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- 应用备注
- Recommended dilution 1:500
- 说明
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A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable.
- 浓度
- 5 μM
- 缓冲液
- Lyophilized from PBS pH 7.4.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light!
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
- 有效期
- 6 months
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 别名
- green fluorescent protein antibody, gfp antibody
- 物质类
- Viral Protein
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