PUM1 抗体
Quick Overview for PUM1 抗体 (ABIN6944728)
抗原
See all PUM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应
- 人, 小鼠
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纯化方法
- Purified by Protein A.
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免疫原
- Recombinant protein within human Pumilio 1 aa 1-200.
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亚型
- IgG
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anti-Pumilio Homolog 1 (PUM1) (AA 1-130) antibody
PUM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-280) antibodyPUM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 62-91) antibodyPUM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB16264 unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 201-300) antibodyPUM1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-1186) antibodyPUM1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (Internal Region) antibodyPUM1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-30), (N-Term) antibodyPUM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB10535 unconjugated
anti-Pumilio Homolog 1 (PUM1) (N-Term) antibodyPUM1 适用: 人 IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (Tyr83) antibodyPUM1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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有效期
- 12 months
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- PUM1 (Pumilio Homolog 1 (PUM1))
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别名
- PUM1
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背景
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Synonyms: Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, PUMH, HSPUM, PUMH1, PUML1, SCA47
Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery. Plays a role in cytoplasmic sensing of viral infection. In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation.
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基因ID
- 9698
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UniProt
- Q14671
抗原
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