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- 抗原 See all PUM1 抗体
- PUM1 (Pumilio Homolog 1 (PUM1))
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PUM1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 特异性
- PUM1 Antibody detects endogenous levels of total PUM1.
- 预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human PUM1, corresponding to a region within the internal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product PUM1 Primary Antibody
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anti-Pumilio Homolog 1 (PUM1) antibody
PUM1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-130) antibodyPUM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-280) antibodyPUM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 62-91) antibodyPUM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB16264 unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 201-300) antibodyPUM1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) antibodyPUM1 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-1186) antibodyPUM1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-30), (N-Term) antibodyPUM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB10535 unconjugated
anti-Pumilio Homolog 1 (PUM1) (Tyr83) antibodyPUM1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (N-Term) antibodyPUM1 适用: 人 IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- PUM1 (Pumilio Homolog 1 (PUM1))
- 别名
- PUM1 (PUM1 产品)
- 别名
- fb96a03 antibody, pumilio1 antibody, si:ch211-285e16.1 antibody, wu:fb96a03 antibody, wu:fb96a08 antibody, zgc:63671 antibody, PUM1 antibody, DKFZp459K0627 antibody, AA517475 antibody, Pumm antibody, mKIAA0099 antibody, HSPUM antibody, PUMH antibody, PUMH1 antibody, PUML1 antibody, pumilio RNA-binding family member 1 antibody, pumilio RNA binding family member 1 antibody, pum1 antibody, PUM1 antibody, Pum1 antibody
- 背景
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Description: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (By similarity). Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233).
Gene: PUM1
- 分子量
- 126 kDa
- 基因ID
- 9698
- UniProt
- Q14671
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