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Recombinant GAPDH 抗体

The 兔 单克隆 anti-GAPDH antibody (ABIN6943517) specifically detects GAPDH in WB, IHC (p), FACS, IHC (fro), IF (p) 和 IF (cc). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN6943517
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant GAPDH 抗体 (ABIN6943517)

抗原

See all GAPDH 抗体
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

抗体类型

Recombinant Antibody

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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单克隆

标记

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This GAPDH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
  • 原理

    GAPDH Recombinant Antibody

    交叉反应

    人, 小鼠, 大鼠

    预测反应

    Zebrafish,Monkey

    纯化方法

    Purified by Protein A.

    亚型

    IgG
  • 应用备注

    WB(WB(1:5000-500000)), FCM(FCM(1 μg/Test)), IHC-P(IHC-P(1:200-1000)), IHC-F(IHC-F(1:200-1000)), IF(IHC-P)(IF(IHC-P)(1:200-1000)), IF(ICC)(IF(ICC)(1:100-500))

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    有效期

    12 months
  • 抗原

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    别名

    GAPDH

    背景

    Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH

    Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

    基因ID

    2597

    UniProt

    P04406
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