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CD34 抗体

CD34 适用: 人, 大鼠 FACS, WB, IF, IHC, StM 宿主: 小鼠 Monoclonal HPCA1-1171 unconjugated
产品编号 ABIN6941172
发货至: 中国
  • 抗原 See all CD34 抗体
    CD34
    适用
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    人, 大鼠
    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD34 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
    特异性
    This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant full-length human HPCA1 protein
    克隆位点
    HPCA1-1171
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD34 Primary Antibody
  • 应用备注

    Positive Control: KG-1 cells. Human tonsil or angiosarcoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    CD34
    别名
    CD34 (CD34 产品)
    别名
    AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
    分子量
    90-110kDa
    基因ID
    947
    UniProt
    P28906
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