CD20 抗体
Quick Overview for CD20 抗体 (ABIN6941106)
抗原
See all CD20 (MS4A1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
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纯化方法
- Purified by Protein A/G
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免疫原
- Lymphoblastoid cell line Daudi
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亚型
- IgG2a kappa
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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (C-Term) antibody
Verified MS4A1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal L26 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 213-297) antibodyMS4A1 适用: 人 FACS, WB, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MS4A1-3409 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rIGEL-773 unconjugated Recombinant Antibody
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 210-297) antibodyMS4A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C11 unconjugated
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应用备注
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Positive Control: Daudi, Raji and U266 and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers.
Known Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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别名
- MS4A1
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分子量
- 33-37kDa
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基因ID
- 931
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UniProt
- P11836
抗原
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