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CD5 抗体 (AA 269-366)

This 小鼠 单克隆 anti-CD5 antibody (Clone CD5-2418) specifically detects CD5 in FACS, IHC, IF 和 StM. The antibody is reactive with 人 samples.
产品编号 ABIN6941057
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD5 抗体 (AA 269-366) (ABIN6941057)

抗原

See all CD5 抗体
CD5

适用

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小鼠

克隆类型

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单克隆

标记

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This CD5 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

克隆位点

CD5-2418
  • 抗原表位

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    AA 269-366

    特异性

    Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)

    亚型

    IgG2c kappa
  • 应用备注

    Positive Control: 293T, Ramos or MOLT-4 cells. Tonsil or Lymph Node.

    Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mg), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CD5

    别名

    CD5

    分子量

    67kDa

    基因ID

    921

    UniProt

    P06127
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