Recombinant Vimentin 抗体
Quick Overview for Recombinant Vimentin 抗体 (ABIN6940885)
抗原
See all Vimentin (VIM) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant full-length human Vimentin protein
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亚型
- IgG
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anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) (AA 435-466), (C-Term) antibodyVIM 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated
anti-Vimentin (VIM) (AA 1-200) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Vimentin (VIM) (AA 41-140) antibodyVIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated
anti-Vimentin (VIM) (AA 210-466) antibodyVIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated
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应用备注
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Positive Control: U-87, A375, Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Vimentin (VIM)
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别名
- VIM
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分子量
- 57-60kDa
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基因ID
- 7431
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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