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Vimentin 抗体 (AA 2-466)

Name: Vimentin 抗体 (AA 2-466)
Brand: antibodies-online
SKU: ABIN6940884
Availability: OutOfStock

This anti-Vimentin antibody is a 小鼠单克隆 antibody detecting Vimentin in IHC 和 StM. Suitable for 人.

Western Blot Analysis of U87 cell lysate using Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

Analysis of Protein Array containing more than 21,000 full-length human proteins using Vimentin Mouse Monoclonal Antibody (VIM/3736) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (Vimentin 抗体 (AA 2-466))

PAr

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

IHC

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

IHC

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

5 images

产品编号 ABIN6940884

发货至: 中国

Datasheet as PDF

Quick Overview for Vimentin 抗体 (AA 2-466) (ABIN6940884)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Immunohistochemistry (IHC), Staining Methods (StM)

克隆位点

VIM-3736

Discover our top product VIM Primary Antibody

抗原表位
32

23

17

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12

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2

2

2

2

2

2

2

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2

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1

1

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1

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1
AA 2-466

特异性

This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

无交叉反应

小鼠, 大鼠

纯化方法

Purified by Protein A/G

免疫原

A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

亚型

IgG2b kappa
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

应用备注

Positive Control: U87, A375, Raji, HEP2 cells. Sarcomas or Melanomas.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

Vimentin (VIM)

别名

VIM

分子量

57-60kDa

基因ID

7431

UniProt

P08670

途径

Caspase Cascade in Apoptosis