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Vimentin 抗体

Name: Vimentin 抗体
Brand: antibodies-online
SKU: ABIN6940879
Availability: OutOfStock

This 小鼠单克隆 anti-Vimentin antibody (Clone VM452) specifically detects Vimentin in WB, IHC, FACS 和 StM. The antibody is reactive with 人, Pig, Cow, 小鸡, 犬, Cat 和山羊 samples.

Western Blot Analysis Raji cell lysate Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin 抗体)

Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (Vimentin 抗体)

PAr

Flow Cytometry of human Vimentin on Jurkat cells. (Vimentin 抗体)

FACS

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin 抗体)

IHC

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin 抗体)

SDS

Western Blot Analysis of human A375 cell lysate using Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin 抗体)

6 images

产品编号 ABIN6940879

发货至: 中国

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中国
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Quick Overview for Vimentin 抗体 (ABIN6940879)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

适用

人, Pig, Cow, 小鸡, 犬, Cat, 山羊

宿主

小鼠

克隆类型

单克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

VM452

Discover our top product VIM Primary Antibody

特异性

This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

无交叉反应

小鼠, 大鼠

纯化方法

Purified by Protein A/G

免疫原

Recombinant full-length human vimentin protein

亚型

IgG1 kappa
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

Vimentin (VIM)

别名

VIM

分子量

57-60kDa

基因ID

7431

UniProt

P08670

途径

Caspase Cascade in Apoptosis