C4B 抗体
Quick Overview for C4B 抗体 (ABIN6940809)
抗原
See all C4B (C4b) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human Complement 4d protein
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亚型
- IgG1 kappa
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anti-Complement Component C4b (C4b) (AA 126-366) antibody
C4b 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 680-756) antibodyC4b 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 1347-1446) antibodyC4b 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1F2 unconjugated
anti-Complement Component C4b (C4b) (AA 19-333) antibodyC4b 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 1454-1744) antibodyC4b 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 144-193) antibodyC4b 适用: 人, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 655-684) antibodyC4b 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: Rejected Renal Transplant Tissue.
Known Application: Immunofluorescence (1:50-1:100), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- C4B (C4b) (Complement Component C4b (C4b))
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别名
- C4B
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分子量
- 192kDa (predicted)
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基因ID
- 720, 721
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途径
- Complement System
抗原
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