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Topoisomerase II alpha 抗体 (AA 1352-1493)

This anti-Topoisomerase II alpha antibody is a 小鼠 单克隆 antibody detecting Topoisomerase II alpha in WB, IHC, IF 和 StM. Suitable for 人 和 小鼠.
产品编号 ABIN6940773
发货至: 中国
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Quick Overview for Topoisomerase II alpha 抗体 (AA 1352-1493) (ABIN6940773)

抗原

See all Topoisomerase II alpha (TOP2A) 抗体
Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

适用

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  • 1
人, 小鼠

宿主

  • 119
  • 38
小鼠

克隆类型

  • 95
  • 62
单克隆

标记

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  • 1
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This Topoisomerase II alpha antibody is un-conjugated

应用范围

  • 111
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  • 1
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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

克隆位点

TOP2A-1361
  • 抗原表位

    • 18
    • 15
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    AA 1352-1493

    特异性

    It recognizes a 170 kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

    亚型

    IgG2b kappa
  • 应用备注

    Positive Control: HeLa cells. Tonsil.

    Known Application: Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

    别名

    TOP2A

    分子量

    170kDa

    基因ID

    7153

    UniProt

    P11388

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases
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