Cyclin D1 抗体
Quick Overview for Cyclin D1 抗体 (ABIN6940486)
抗原
See all Cyclin D1 (CCND1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40 % of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
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纯化方法
- Purified by Protein A/G
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免疫原
- Human full length recombinant cyclin D1 protein
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亚型
- IgG2a kappa
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anti-Cyclin D1 (CCND1) (AA 61-110) antibody
CCND1 适用: 人, 小鼠, 大鼠, Cow, 兔, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) antibodyKO Validated CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Cyclin D1 (CCND1) (AA 167-295) antibodyCCND1 适用: 人 IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3B6G3 unconjugated
anti-Cyclin D1 (CCND1) (AA 1-295) antibodyCCND1 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1F7F5 unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (AA 68-97) antibodyCCND1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB8066 unconjugated
anti-Cyclin D1 (CCND1) (AA 68-97) antibodyCCND1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (AA 1-233) antibodyCCND1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cyclin D1 (CCND1)
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别名
- CCND1
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分子量
- 36kDa
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基因ID
- 595
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UniProt
- P24385
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途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
抗原
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