Recombinant KRT20 抗体 (AA 196-323)
Quick Overview for Recombinant KRT20 抗体 (AA 196-323) (ABIN6940352)
抗原
See all KRT20 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 196-323
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特异性
- This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
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Positive Control: HT29 cell lysate (WB). Human colon carcinoma (IHC).
Known Application: Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- KRT20 (Keratin 20 (KRT20))
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别名
- KRT20
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分子量
- 46kDa
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基因ID
- 54474
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UniProt
- P35900
抗原
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