Prohibitin 抗体 (AA 167-261)
Quick Overview for Prohibitin 抗体 (AA 167-261) (ABIN6940301)
抗原
See all Prohibitin (PHB) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 167-261
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特异性
- Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)
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亚型
- IgG1 kappa
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anti-Prohibitin (PHB) (AA 1-272) antibody
PHB 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F4-2B2 unconjugated
anti-Prohibitin (PHB) antibodyKO Validated PHB 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin (PHB) antibodyPHB 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5H7 unconjugated
anti-Prohibitin (PHB) (AA 177-272) antibodyPHB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin (PHB) (AA 167-261) antibodyPHB 适用: 人 WB, IHC, IF, StM 宿主: 小鼠 Monoclonal PHB-3194 unconjugated
anti-Prohibitin (PHB) (AA 73-272) antibodyPHB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Prohibitin (PHB) (AA 167-261) antibodyPHB 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal PHB-3230 unconjugated
anti-Prohibitin (PHB) (AA 167-261) antibodyPHB 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal PHB-3193 unconjugated
anti-Prohibitin (PHB) (AA 167-261) antibodyPHB 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal PHB-1881 unconjugated
anti-Prohibitin (PHB) (AA 89-117) antibodyPHB 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB17513 unconjugated
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应用备注
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Positive Control: Ramos, Jurkat, HEK293, K562, HepG2, A431 or MCF-7 cells (IF). Liver, Kidney, Heart or Endometrium (IHC).
Known Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Prohibitin (PHB)
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别名
- PHB
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分子量
- 30kDa
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基因ID
- 5245
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UniProt
- P35232
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
抗原
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