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NUMA1 抗体

This anti-NUMA1 antibody (ABIN6940233) is a Mouse Monoclonal antibody detecting NUMA1 in IHC, IF, FACS, StM. Suitable for Human.
产品编号 ABIN6940233
发货至: 中国

Quick Overview for NUMA1 抗体 (ABIN6940233)

抗原

See all NUMA1 抗体
NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This NUMA1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

A73-B-D12
  • 特异性

    Recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

    免疫原

    Colon carcinoma 174T cells

    亚型

    IgM kappa
  • 应用备注

    Positive Control: Exponentially growing any cultured human cells. Tonsil or lymph node.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM citrate buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    200 μg/mL of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

    别名

    NUMA1

    分子量

    228kDa

    基因ID

    4926

    UniProt

    Q14980

    途径

    Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase
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