NEFL 抗体
Quick Overview for NEFL 抗体 (ABIN6940187)
抗原
See all NEFL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Crude neurofilament preparation from porcine spinal cord
-
亚型
- IgG
-
-
anti-Neurofilament, Light Polypeptide (NEFL) antibody
NEFL 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 400-543) antibodyNEFL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-360) antibodyNEFL 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3G2 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-284) antibodyNEFL 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 128-271) antibodyNEFL 适用: 人 IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: Pig WB, IF 宿主: 小鼠 Monoclonal DA2 unconjugated
-
-
应用备注
-
Positive Control: HEK293 cells, Brain, Neuroblastoma.
Known Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- NEFL (Neurofilament, Light Polypeptide (NEFL))
-
别名
- NEFL
-
分子量
- 68kDa
-
基因ID
- 4747
-
UniProt
- P07196
抗原
-
