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NEFH 抗体

This anti-NEFH antibody is a 小鼠 单克隆 antibody detecting NEFH in WB, FACS, ELISA, IHC (fro), StM 和 Coat. Suitable for 人, 大鼠, 小鼠, 小鸡 和 Pig.
产品编号 ABIN6940181
发货至: 中国

Quick Overview for NEFH 抗体 (ABIN6940181)

抗原

See all NEFH 抗体
NEFH (Neurofilament, Heavy Polypeptide (NEFH))

适用

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人, 大鼠, 小鼠, 小鸡, Pig

宿主

  • 70
  • 63
  • 9
小鼠

克隆类型

  • 88
  • 54
单克隆

标记

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This NEFH antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Staining Methods (StM), Coating (Coat)

克隆位点

SPM563
  • 特异性

    This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

    纯化方法

    Purified by Protein A/G

    免疫原

    Human recombinant neurofilament protein

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: Brain, Neuroblastoma.

    Known Application: ELISA (For coating, order Ab without BSA), Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Frozen & formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    NEFH (Neurofilament, Heavy Polypeptide (NEFH))

    别名

    NEFH

    分子量

    200kDa

    基因ID

    4744

    UniProt

    P12036
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