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- 抗原 See all MLH1 抗体
- MLH1 (MutL Homolog 1 (MLH1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MLH1 antibody is un-conjugated
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应用范围
- ELISA, Coating (Coat)
- 特异性
- This MAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human MLH1 protein
- 克隆位点
- MLH1-1324
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product MLH1 Primary Antibody
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anti-MutL Homolog 1 (MLH1) (AA 1-756) antibody
MLH1 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal M1 unconjugated
anti-MutL Homolog 1 (MLH1) (AA 381-483) antibodyMLH1 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 8C12B7 unconjugated
anti-MutL Homolog 1 (MLH1) (AA 381-483) antibodyMLH1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A7C8 unconjugated
anti-MutL Homolog 1 (MLH1) (AA 350-519) antibodyMLH1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (AA 381-483) antibodyMLH1 适用: 人, 猴 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 4C9C7 unconjugated
anti-MutL Homolog 1 (MLH1) (Internal Region) antibodyMLH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) antibodyMLH1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (AA 501-600) antibodyMLH1 适用: 人 ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (Internal Region) antibodyMLH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (AA 373-735) antibodyMLH1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: Molt-4, HeLa or Jurkat cells. Lymph node or Colon, breast, lung, testis, prostate or thyroid carcinoma.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- MLH1 (MutL Homolog 1 (MLH1))
- 别名
- MLH1 (MLH1 产品)
- 别名
- CG11482 antibody, Dmel\\CG11482 antibody, dmlh-1 antibody, dmlh1 antibody, zgc:66301 antibody, MLH1 antibody, LOC100232198 antibody, 1110035C23Rik antibody, AI317206 antibody, AI325952 antibody, AI561766 antibody, COCA2 antibody, FCC2 antibody, HNPCC antibody, HNPCC2 antibody, hMLH1 antibody, mutL homolog 1 antibody, CG11482 gene product from transcript CG11482-RB antibody, mutL homolog 1 S homeolog antibody, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody, DNA mismatch repair protein Mlh1 antibody, MLH1 antibody, Mlh1 antibody, mlh1.S antibody, mlh1 antibody, LOC588545 antibody
- 分子量
- 85kDa
- 基因ID
- 4292
- UniProt
- P40692
- 途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
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