Ki-67 抗体 (AA 2293-2478)
Quick Overview for Ki-67 抗体 (AA 2293-2478) (ABIN6940053)
抗原
See all Ki-67 (MKI67) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 2293-2478
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特异性
- Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)
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亚型
- IgG2b kappa
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应用备注
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Positive Control: Any actively proliferating cells. Skin, Tonsil or Lymph Node.
Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
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别名
- MKI67
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分子量
- 345kDa and 395kDa
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基因ID
- 4288
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UniProt
- P46013
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途径
- Glycosaminoglycan Metabolic Process
抗原
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