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KRT14 抗体 (AA 351-472)

This anti-KRT14 antibody is a 小鼠 单克隆 antibody detecting KRT14 in IHC, FACS 和 StM. Suitable for 人, 大鼠, 小鼠 和 Pig.
产品编号 ABIN6939908
发货至: 中国

Quick Overview for KRT14 抗体 (AA 351-472) (ABIN6939908)

抗原

See all KRT14 抗体
KRT14 (Keratin 14 (KRT14))

适用

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人, 大鼠, 小鼠, Pig

宿主

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小鼠

克隆类型

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单克隆

标记

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This KRT14 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

KRT14-2375
  • 抗原表位

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    AA 351-472

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant human KRT14 fragment (around aa351-472) (exact sequence is proprietary)

    亚型

    IgG2a kappa
  • 应用备注

    Positive Control: A431 or HeLa cells. Skin or Squamous Cell Carcinoma.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    KRT14 (Keratin 14 (KRT14))

    别名

    KRT14

    背景

    Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

    分子量

    50kDa

    基因ID

    3861

    UniProt

    P02533
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