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Cytokeratin 7 抗体

This anti-Cytokeratin 7 antibody is a 兔 单克隆 antibody detecting Cytokeratin 7 in WB, IHC, FACS, StM 和 ISt. Suitable for 人.
产品编号 ABIN6939879
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for Cytokeratin 7 抗体 (ABIN6939879)

抗原

See all Cytokeratin 7 (KRT7) 抗体
Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

适用

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克隆类型

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单克隆

标记

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This Cytokeratin 7 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM), Immunostaining (ISt)

克隆位点

KRT7-1499R
  • 特异性

    It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

    免疫原

    Recombinant full-length human KRT7 protein

    亚型

    IgG
  • 应用备注

    Positive Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

    别名

    KRT7

    分子量

    55kDa

    基因ID

    3855

    UniProt

    P08729
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