Cytokeratin 5 抗体 (AA 316-590)
Quick Overview for Cytokeratin 5 抗体 (AA 316-590) (ABIN6939864)
抗原
See all Cytokeratin 5 (KRT5) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 316-590
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特异性
- This MAb recognizes a protein of 58 kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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应用备注
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Positive Control: MCF-7 or HeLa cells. Human tonsil, esophagus or bladder tissue (IHC).
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))
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别名
- KRT5
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分子量
- 58kDa
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基因ID
- 3852
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UniProt
- P13647
抗原
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