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PD-L1 抗体 (AA 39-191)

This anti-PD-L1 antibody is a 小鼠 单克隆 antibody detecting PD-L1 in WB, FACS, IHC, ELISA, IF, Coat 和 StM. Suitable for 人 和 小鼠.
产品编号 ABIN6939571
发货至: 中国

Quick Overview for PD-L1 抗体 (AA 39-191) (ABIN6939571)

抗原

See all PD-L1 抗体
PD-L1 (CD274 (PD-L1))

适用

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人, 小鼠

宿主

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  • 1
小鼠

克隆类型

  • 203
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  • 7
单克隆

标记

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This PD-L1 antibody is un-conjugated

应用范围

  • 193
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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

克隆位点

PDL1-2746
  • 抗原表位

    • 71
    • 29
    • 16
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    • 11
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    AA 39-191

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)

    亚型

    IgG2b kappa
  • 应用备注

    Positive Control: Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat cells.

    Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    PD-L1 (CD274 (PD-L1))

    别名

    CD274

    背景

    PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

    分子量

    37-50kDa

    基因ID

    29126

    UniProt

    Q9NZQ7

    途径

    Cancer Immune Checkpoints
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