PD-L1 抗体 (AA 39-191)
Quick Overview for PD-L1 抗体 (AA 39-191) (ABIN6939571)
抗原
See all PD-L1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 39-191
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
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亚型
- IgG2b kappa
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应用备注
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Positive Control: Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat cells.
Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- PD-L1 (CD274 (PD-L1))
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别名
- CD274
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背景
- PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
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分子量
- 37-50kDa
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基因ID
- 29126
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UniProt
- Q9NZQ7
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途径
- Cancer Immune Checkpoints
抗原
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