FBXO5 抗体 (AA 1-250)
Our Local Distributor
北京 101111
Quick Overview for FBXO5 抗体 (AA 1-250) (ABIN6939501)
抗原
See all FBXO5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 1-250
-
特异性
- It recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
-
亚型
- IgG2a kappa
-
-
anti-F-Box Protein 5 (FBXO5) (C-Term) antibody
FBXO5 适用: 人, 犬, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 326-375) antibodyFBXO5 适用: 人, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5H7 unconjugated
anti-F-Box Protein 5 (FBXO5) (C-Term) antibodyFBXO5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 1-250) antibodyFBXO5 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal EMI1-1176 unconjugated
-
-
应用备注
-
Positive Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.
Known Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- FBXO5 (F-Box Protein 5 (FBXO5))
-
别名
- FBXO5
-
分子量
- 56kDa
-
基因ID
- 26271
-
UniProt
- Q9UKT4
-
途径
- Mitotic G1-G1/S Phases
抗原
-
