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MLANA 抗体

This 小鼠 单克隆 anti-MLANA antibody (Clone A103-M2-7C10-M2-9E3) specifically detects MLANA in WB, IF, FACS, IHC, ISt 和 StM. The antibody is reactive with 人, 小鼠, 大鼠 和 犬 samples.
产品编号 ABIN6939396
发货至: 中国
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Quick Overview for MLANA 抗体 (ABIN6939396)

抗原

See all MLANA 抗体
MLANA (Melan A (MLANA))

适用

  • 214
  • 115
  • 90
  • 35
  • 16
  • 1
人, 小鼠, 大鼠, 犬

宿主

  • 110
  • 103
  • 1
  • 1
小鼠

克隆类型

  • 140
  • 75
单克隆

标记

  • 76
  • 17
  • 9
  • 9
  • 9
  • 9
  • 9
  • 9
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  • 5
  • 5
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  • 2
  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
This MLANA antibody is un-conjugated

应用范围

  • 147
  • 110
  • 109
  • 84
  • 77
  • 53
  • 26
  • 26
  • 23
  • 23
  • 16
  • 13
  • 11
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  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

克隆位点

A103-M2-7C10-M2-9E3
  • 特异性

    This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant full-length hMART-1 protein (A103, M2-7C10, M2-9E3)

    亚型

    IgG2a kappa
  • 应用备注

    Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    MLANA (Melan A (MLANA))

    别名

    MLANA

    分子量

    20-22kDa (doublet)

    基因ID

    2315

    UniProt

    Q16655
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