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EPO 抗体 (AA 28-162)

This 小鼠 单克隆 anti-EPO antibody (Clone EPO-1367) specifically detects EPO in IHC, ELISA, Coat 和 StM. The antibody is reactive with 人 samples.
产品编号 ABIN6939312
发货至: 中国
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Quick Overview for EPO 抗体 (AA 28-162) (ABIN6939312)

抗原

See all EPO 抗体
EPO (Erythropoietin (EPO))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This EPO antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

克隆位点

EPO-1367
  • 抗原表位

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    AA 28-162

    特异性

    Recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant fragment (around aa 28-162) of human EPO protein (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: HepG2 cells. Heart or Kidney.

    Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    EPO (Erythropoietin (EPO))

    别名

    EPO

    物质类

    Hormone

    分子量

    37kDa

    基因ID

    2056

    UniProt

    P01588

    途径

    JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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