Desmin 抗体
Quick Overview for Desmin 抗体 (ABIN6939233)
抗原
See all Desmin (DES) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human desmin protein
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亚型
- IgG1 kappa
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anti-Desmin (DES) (C-Term) antibody
Verified DES 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Desmin (DES) (AA 1-304) antibodyDES 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) (AA 261-360) antibodyDES 适用: 人, 大鼠, 小鼠, 小鸡 WB, ELISA, IHC (p), FACS, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) antibodyDES 适用: 人 WB, IF, IHC (p), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) (AA 121-470) antibodyDES 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) antibodyDES 适用: 人, 大鼠, 小鼠, 小鸡, Hamster, Pig, 犬, 兔, 斑马鱼 WB, IHC, IHC (fro) 宿主: 小鼠 Monoclonal RD301 unconjugated
anti-Desmin (DES) antibodyDES 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal 3E1G4 unconjugated
anti-Desmin (DES) (C-Term) antibodyDES 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) (AA 361-470) antibodyDES 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D12 unconjugated
anti-Desmin (DES) antibodyDES 适用: 人, 大鼠, 小鼠, Hamster, Pig, 犬, 兔 WB, IHC, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Positive Control: SJRH30 cells. Uterus or Leiomyosarcoma.
Known Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Desmin (DES)
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别名
- DES
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分子量
- 52kDa
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基因ID
- 1674
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UniProt
- P17661
抗原
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