CLTA/CLTB 抗体 (N-Term)
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北京 101111
Quick Overview for CLTA/CLTB 抗体 (N-Term) (ABIN6939151)
抗原
See all CLTA/CLTB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
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纯化方法
- Purified by Protein A/G
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免疫原
- Purified recombinant N-terminal fragment of human Clathrin Light Chain
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亚型
- IgG2b kappa
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anti-Clathrin, Light Chain A/B (CLTA/CLTB) (N-Term) antibody
CLTA/CLTB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CLC-1421 unconjugated
anti-Clathrin, Light Chain A/B (CLTA/CLTB) (C-Term) antibodyCLTA/CLTB 适用: 人, 小鼠, 大鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: HeLa cells. Placenta or Prostate Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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