CLTA/CLTB 抗体 (N-Term)
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- 抗原 See all CLTA/CLTB 抗体
- CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CLTA/CLTB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Purified recombinant N-terminal fragment of human Clathrin Light Chain
- 克隆位点
- SPM174
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CLTA/CLTB Primary Antibody
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anti-Clathrin, Light Chain A/B (CLTA/CLTB) (N-Term) antibody
CLTA/CLTB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CLC-1421 unconjugated
anti-Clathrin, Light Chain A/B (CLTA/CLTB) (N-Term) antibodyCLTA/CLTB 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal SPM174 unconjugated
anti-Clathrin, Light Chain A/B (CLTA/CLTB) (C-Term) antibodyCLTA/CLTB 适用: 人, 小鼠, 大鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Placenta or Prostate Carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))
- 别名
- CLTA and CLTB (CLTA/CLTB 产品)
- 别名
- lca antibody, zgc:73358 antibody, wu:fb61d08 antibody, wu:fj48d06 antibody, LCA antibody, clc antibody, DDBDRAFT_0169251 antibody, DDBDRAFT_0191305 antibody, DDB_0169251 antibody, DDB_0191305 antibody, clcb antibody, clathrin light chain A antibody, clathrin, light chain A antibody, Clathrin light chain antibody, clathrin light chain antibody, clathrin light chain B antibody, Clathrin light chain B antibody, CLTA antibody, clta antibody, clic-1 antibody, CLC1 antibody, clc antibody, CLTB antibody, clcb antibody, CNI00580 antibody, LOC5567495 antibody, CpipJ_CPIJ018056 antibody, PTRG_01988 antibody, PMAA_096950 antibody, SJAG_04517 antibody, TSTA_044110 antibody, PAS_chr1-4_0158 antibody
- 背景
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Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), CLTA, CLTB,Clathrin, Light Chain
Cellular localisation: Cytoplasmic - 分子量
- 31-44kDa
- 基因ID
- 1211, 1212, 484241
- UniProt
- P09496, P09497
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