N-Cadherin 抗体 (Extracellular Domain)
Quick Overview for N-Cadherin 抗体 (Extracellular Domain) (ABIN6939013)
抗原
See all N-Cadherin (CDH2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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特异性
- Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
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交叉反应 （详细）
- Human and Mouse. Others not known.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant human N-cadherin extracellular domain
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亚型
- IgG1
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anti-Cadherin 2 (CDH2) (AA 701-800) antibody
CDH2 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人, 小鼠, 犬, 猴 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2G7 unconjugated
anti-Cadherin 2 (CDH2) (AA 750-850) antibodyCDH2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 5D5 unconjugated
anti-Cadherin 2 (CDH2) (AA 45-238) antibodyCDH2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (AA 685-784) antibodyCDH2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (AA 295-414) antibodyCDH2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人 WB, IHC, IHC (f) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Cadherin 2 (CDH2) (AA 701-714), (Middle Region) antibodyCDH2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (C-Term) antibodyCDH2 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: HeLa or HUVEC cells. Human heart, pancreas or cerebral cortex (IHC).
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blotting (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tis with1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
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有效期
- 24 months
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- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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别名
- CDH2
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背景
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Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD,N-Cadherin / Cadherin-2 / CD325 (NCAD)
Cellular localisation: Cell surface -
分子量
- 130-140kDa
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基因ID
- 1000, 464829
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UniProt
- P19022
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途径
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
抗原
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