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VWF 抗体 (AA 845-949)

VWF 适用: 人 WB, FACS, IP, IF, IHC (f) 宿主: 小鼠 Monoclonal 3E2D10 unconjugated
产品编号 ABIN6938770
发货至: 中国
  • 抗原 See all VWF 抗体
    VWF (Von Willebrand Factor (VWF))
    抗原表位
    • 31
    • 16
    • 10
    • 10
    • 7
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 845-949
    适用
    • 150
    • 39
    • 26
    • 6
    • 5
    • 3
    宿主
    • 86
    • 77
    • 16
    • 9
    小鼠
    克隆类型
    • 94
    • 91
    • 1
    单克隆
    标记
    • 94
    • 21
    • 15
    • 9
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This VWF antibody is un-conjugated
    应用范围
    • 109
    • 101
    • 71
    • 54
    • 51
    • 51
    • 25
    • 18
    • 16
    • 15
    • 14
    • 9
    • 9
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
    交叉反应 (详细)
    Human.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Recombinant human vWF fragment spanning around aa 845-949 (exact sequence is proprietary)
    克隆位点
    3E2D10
    亚型
    IgG1 kappa
    Top Product
    Discover our top product VWF Primary Antibody
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein lysate),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HUVEC cells. Tonsil.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    VWF (Von Willebrand Factor (VWF))
    别名
    VWF (VWF 产品)
    别名
    VWF antibody, si:ch1073-474e24.1 antibody, F8VWF antibody, VWD antibody, 6820430P06Rik antibody, AI551257 antibody, B130011O06Rik antibody, C630030D09 antibody, von Willebrand factor antibody, Von Willebrand factor antibody, VWF antibody, vwf antibody, Vwf antibody
    背景
    Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD),von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)
    Cellular localisation: Cytoplasmic
    分子量
    250kDa
    基因ID
    7450, 440848
    UniProt
    P04275
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