Ubiquitin B 抗体 (AA 1-119)
Quick Overview for Ubiquitin B 抗体 (AA 1-119) (ABIN6938717)
抗原
See all Ubiquitin B (UBB) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-119
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特异性
- Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)
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亚型
- IgG2c kappa
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anti-Ubiquitin B (UBB) (AA 1-119) antibody
UBB 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal UBB-1748 unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB 适用: 人 WB, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal UBB-2122 unconjugated
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3C12 unconjugated
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-76) antibodyUBB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Ubiquitin B (UBB) (AA 77-152) antibodyUBB 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 103-228) antibodyUBB 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB 适用: 人 IHC, StM 宿主: 兔 Monoclonal UBB-3143R unconjugated Recombinant Antibody
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin B (UBB) (AA 25-57), (N-Term) antibodyUBB 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB49507 unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, HeLa or Jurkat cells. Alzheimer's Brain.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Ubiquitin B (UBB)
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别名
- UBB
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背景
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Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2,Ubiquitin (Autophagy Marker)
Cellular localisation: Cell Surface, Cytoplasmic and Nuclear -
分子量
- 9kDa
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基因ID
- 7314, 356190, 520348
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UniProt
- P62979, P62987, P62988
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway
抗原
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