TCF4 抗体 (AA 365-671)
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Quick Overview for TCF4 抗体 (AA 365-671) (ABIN6938516)
抗原
See all TCF4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 365-671
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原理
- TCF4 (Transcription Factor 4) Antibody
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特异性
- Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.
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交叉反应 (详细)
- Human.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)
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亚型
- IgG2a, kappa
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or HepG2 cells. Human bladder, colon or tonsil.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- TCF4 (Transcription Factor 4 (TCF4))
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别名
- TCF4
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背景
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bHLHb19, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, PTHS, SEF2, SL3-3 enhancer factor 2, TCF4, Transcription factor 4
colon or tonsil., HeLa or HepG2 cells. Human bladder
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分子量
- 60kDa
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基因ID
- 6925, 605153
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UniProt
- P15884
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途径
- WNT signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus
抗原
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