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TCF4 抗体 (AA 365-671)

This anti-TCF4 antibody is a 小鼠 单克隆 antibody detecting TCF4 in WB, ELISA, Coat 和 IHC (f). Suitable for 人.
产品编号 ABIN6938516
发货至: 中国

Quick Overview for TCF4 抗体 (AA 365-671) (ABIN6938516)

抗原

See all TCF4 抗体
TCF4 (Transcription Factor 4 (TCF4))

适用

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宿主

  • 52
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  • 1
小鼠

克隆类型

  • 51
  • 19
单克隆

标记

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This TCF4 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

TCF4-1705
  • 抗原表位

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    AA 365-671

    特异性

    Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)

    亚型

    IgG2a kappa
  • 应用备注

    Known_Application: ELISA (For coating, order antibody without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or HepG2 cells. Human bladder, colon or tonsil.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    TCF4 (Transcription Factor 4 (TCF4))

    别名

    TCF4

    背景

    BHLHb19, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, PTHS, SEF2, SL3-3 enhancer factor 2, TCF4, Transcription factor 4,TCF4 (Transcription Factor 4)
    Cellular localisation: Nucleus. Cytoplasm.

    分子量

    60kDa

    基因ID

    6925, 605153

    UniProt

    P15884

    途径

    WNT signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus
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