OGG1 抗体
Quick Overview for OGG1 抗体 (ABIN6938109)
抗原
See all OGG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- 8-oxoguanine (8-oxoG), an oxidized form of guanine, is produced by reactive oxygen species in both DNA and nucleotide pools during normal aging. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversionmutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. OGG1 (for 8-oxoG DNA glycosylase), also designated MMH, is a DNA repair enzyme that corrects these mutations. Inactivation of the OGG1 gene leads to a mutator phenotype, characterized by the increase in G:C to T:A transversions. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Guanine is the main target for reactive oxygen species in DNA, and 8-oxoguanine is the most frequent base lesion. Therefore, formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. Furthermore, defects in OGG1 may be a cause of renal cell carcinoma.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human OGG1 protein
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亚型
- IgG2c kappa
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anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 1-100), (N-Term) antibody
OGG1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 31-345) antibodyOGG1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 151-250) antibodyOGG1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 1-345) antibodyOGG1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 1-324) antibodyOGG1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 1-324) antibodyOGG1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) (Internal Region) antibodyVerified OGG1 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) antibodyOGG1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CPTC-OGG1-1 unconjugated
anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 31-345) antibodyOGG1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or Jurkat cells. Kidney, skin or lymph node.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))
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别名
- OGG1
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背景
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HMMH, HOGG1, MUTM, OGH1,8-Oxoguanine DNA Glycosylase
Cellular localisation: Mitochondrion. Nucleus speckle. -
分子量
- 39kDa
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基因ID
- 4968, 380271
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UniProt
- O15527
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途径
- DNA Damage Repair
抗原
-
